Antibodies and antisera used in the immunohistochemical study
Antibody | Specificity | Target | Source | Pretreatment and dilution |
---|---|---|---|---|
MW, microwave oven. | ||||
UCHL-1 | Human antigen CD45RO | T lymphocytes | Dako | None, 1:200 |
PGM-1 | Human antigen CD68 | Macrophages | Dako | 10′ Trypsin 37°C, 1:40 |
Glycophorin-A | Glycophorin-A | Erythrocytes | Dako | None, 1:100 |
QBEND/10 | Human antigen CD34 | Endothelia | Serotec | None, 1:100 |
HHF35 | α and γ isotypes of skeletal, cardiac, and smooth muscle actin | Smooth muscle cells | Enzo Diagnostic | None, 1:100 |
CD41a | IIIa platelet glycoprotein | Platelets | Immunotech | 20′ Subtilysin 37°C, 1:100 |
CD61 | IIb/IIIa platelet glycoprotein | Platelets | Immunotech | 2 × 5′ MW 500 W, 1:50 |
Fibrinogen | Fibrinogen and fibrin | Fibrin network | Biogenex | None, 1:200 |