Table 1

 Oligonucleotides

LengthGC (%)Tm (°C)
Human cytokine coding genes (GenBank Accession Numbers: TNFα (M10988), IL-1β (M15330), IL-6 (M29150), β actin (X00351).
IL, interleukin; Tm, melting temperature; TNFα, tumour necrosis factor α.
TNFα
Primers
    5′-AAGAGTCCCCCAGGGACCTCM-3′216153
    5′-CCTGGGAGTAGATGAGGTACA-3′215249
Product23088.2
IL-6
Primers
    5′-CAGCTATGAACTCCTTCTCCACAAGC-3′265064.8
    5′-CTGAGATGCCGTCGAGGATGTACCG-3′256067.9
Product20185.5
β Actin
Primers
    5′-AGCACGGCATCGTCACCAACT-3′2157.162.7
    5′-TGGCTGGGGTGTTGAAGGTCT-3′2157.162.7
Product18086.0