Peptide substrates of NEP28
| Peptide | Km (μM) | Kcat(min−1) | Kcat/Km (min−1 μM−1) |
| β-amyloid protein 42 | 2.8 | – | – |
| Proadrenomedullin | 6.1 | – | – |
| ANP28 | 28.3 | 145 | 5.1 |
| Substance P | 31.9 | 5062 | 158.7 |
| Bradykinin | 92.2 | 6364 | 69.0 |
| BNP32 | 102 | 54.3 | 0.53 |
| Angiotensin II | 280 | – | – |
ANP, atrial natriuretic peptide; Km, Michaelis constant is the concentration of a substrate which allows the enzyme to achieve half of maximum reaction velocity, it is the inverse measure of affinity of an enzyme to its substrate (ie, lower the Km, the higher the affinity of an enzyme to the substrate); Kcat, catalytic production rate under optimum conditions; Kcat/Km, comparison ratio that allows for evaluation of the efficiency of an enzyme on different substrates; NEP, neprilysin.