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Th17 Cells Facilitate the Humoral Immune Response in Patients with Acute Viral Myocarditis

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Abstract

Background

Recently, the Th17 cell, a newly determined CD4+Th subset, was reported to participate in the inflammation of myocarditis combined with Th1 cells, and this study aimed to explore whether it was involved in the Th2 cell-mediated humoral immunity in viral myocarditis.

Methods

A total of 34 patients, including 16 acute viral myocarditis (AVMC) and 18 dilated cardiomyopathy (DCM) having a history of AVMC, were enrolled for this study besides 18 healthy volunteers.

Results

The frequencies of Th17 and Th1 cells, especially Th17 cells in AVMC patients, while those of Th1 and Th2 cells, especially Th2 cells in DCM group, were all increased significantly compared with those in healthy volunteers (P < 0.01), with no changes of Th2 cells in AVMC and Th17 cells in DCM groups. The similar results were also observed in Th cell cytokines (IL-17, INF-γ, and IL-4) and key transcript factors (RORγt, T-bet, and GATA-3). Meanwhile, antiheart antibodies (AHA) of IgG type were found in 15 (93.8%) patients with AVMC and ten (55.6%) cases with DCM, accompanied by the higher expression of IL-17R on B cells and the frequencies of B cells than those in healthy controls (P < 0.01 in AVMC and P < 0.05 in DCM, respectively) who had no AHA. Furthermore, both of the B cell activities in AVMC and DCM groups were elevated and positively correlated to serum IL-17 (R = 0.66, P < 0.01) and IL-4 (R = 0.47, P < 0.05) respectively, with no correlation to INF-γ.

Conclusions

It was Th17 cells but not Th2 cells that helped the B cells to produce AHA in AVMC and not until at the late phase of viral myocarditis could Th2 cells play the important role in mediating humoral response.

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Acknowledgment

The work described in this article was supported by grants from National Basic Research Program of China (973 Program): 2007CB512000; 2007CB512005, the Key Laboratory of the Chinese Ministry (number 2007A01) and the Hubei Province Natural Science Foundation of China (number 2008CDB147).

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Authors and Affiliations

  1. Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China

    Jing Yuan, Ai-Lin Cao, Miao Yu, Qiong-Wen Lin, Xian Yu, Min Wang, He-Ping Guo & Yu-Hua Liao

  2. Laboratory of Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China

    Jing-Hui Zhang

  3. Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Jie-Fang Avenue 1277, Wuhan, 430022, China

    Yu-Hua Liao

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  1. Jing Yuan
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  2. Ai-Lin Cao
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Correspondence to Yu-Hua Liao.

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Yuan, J., Cao, AL., Yu, M. et al. Th17 Cells Facilitate the Humoral Immune Response in Patients with Acute Viral Myocarditis. J Clin Immunol 30, 226–234 (2010). https://doi.org/10.1007/s10875-009-9355-z

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  • DOI: https://doi.org/10.1007/s10875-009-9355-z

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