Elsevier

Cytokine

Volume 6, Issue 1, January 1994, Pages 87-91
Cytokine

Original contribution
Interleukin 6 gene transcripts are expressed in human atherosclerotic lesions

https://doi.org/10.1016/1043-4666(94)90013-2Get rights and content

Abstract

Factors controlling the proliferation of vascular smooth muscle cells (SMC) are thought to be key elements in the progression of atherosclerosis. We have previously shown that interleukin 6 (IL-6) stimulates the growth of SMC in vitro and that IL-6 gene transcripts are expressed in atherosclerotic lesions of genetically hyperlipidemic rabbits. To understand the involvement of IL-6 in the development of human atherosclerosis, we investigated IL-6 mRNA expression in atherosclerotic arteries from patients undergoing surgical vascularization, utilizing reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization analyses. In RT-PCR analysis, the atherosclerotic arteries showed 10- to 40-fold levels of IL-6 mRNA expression over the non-atherosclerotic artery. In in situ hybridization analysis, IL-6 gene transcripts were observed in the thickened intimal layer of atherosclerotic lesions. These results strongly suggest the involvement of IL-6 in the development of human atherosclerosis.

References (21)

  • U Ikeda et al.

    Interleukin 6 gene transcripts are expressed in atherosclerotic lesions of genetically hyperlipidemic rabbits

    Atherosclerosis

    (1992)
  • T Hirano et al.

    Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin

    Nature

    (1986)
  • GK Hanson et al.

    Detection of activated T lymphocytes in human atherosclerotic plaque

    Am J Physiol

    (1989)
  • U Ikeda et al.

    Interleukin-6 stimulates the growth of vascular cells in a PDGF-dependent manner

    Am J Physiol

    (1991)
  • Y Seino et al.

    Increased IL-6 mRNA expression in cardiac myxomas

    B Heart J

    (1993)
  • PR Muller et al.

    Rapid preparation of crude RNA

    PR Muller et al.

    Rapid preparation of crude RNA

  • C Chiu et al.

    Multiple biological activities are expressed by a mouse interleukin 6 cDNA clone isolated from bone marrow stromal cells

  • R Ross et al.

    Localization of PDGF-B protein in macrophages in all phases of atherogenesis

    Science

    (1990)
  • T Barrett et al.

    Platelet-derived growth factor gene expression in human atherosclerotic plaques and normal artery

  • JN Wilcox et al.

    Platelet-derived growth factor mRNA detection in human atherosclerotic plaques by in situ hybridization

    J Clin Invest

    (1988)
There are more references available in the full text version of this article.

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