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Cardiac chamber-specific alterations of ANP and BNP expression with advancing age and with systemic hypertension

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Abstract

The present study determined cardiac chamber-specific alterations of the expression of the atrial and brain natriuretic peptide (ANP and BNP) genes with a small increase in age beyond adulthood and with systemic hypertension of intermediate duration. The expression distributions of these genes was determined using in situ hybridization in the right and left atria (RA and LA), and the right and left ventricles (RV and LV) in Wistar Kyoto rats (WKY) and age-matched Spontaneously Hypertensive rats (SHR) at ages 6 months (adult) and 8 months (advanced-age beyond adulthood).

In all rat groups, both genes were expressed (ANP > BNP) in the LA and LV, and were not expressed in the RA and RV. The genes were expressed in the LA in all rat groups; the ANP, but not the BNP, expression increased with advancing age and with superimposed hypertension. They were expressed in the LV of the advanced-age WKY, adult and advanced-age SHR, but not in the adult WKY. The ANP mRNA labeling in the LA was diffuse and interspersed with dense accumulations, whereas BNP labeling was diffuse. The labeling of both genes in the form of sparse clusters was seen in the LV of the advanced-age SHR. Our study showed that ANP and BNP expression in left heart chambers increased with a small increase in age, with hypertension of intermediate duration, and with modest left ventricular hypertrophy. The chamber-specific expression distribution could be due to special groups of cardiac cells, or to local chamber-specific factors.

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References

  1. Arai H, Nakao K, Saito Y, Morii N, Sugawara A, Yamada T, Itoh H, Shiono S, Mukoyama M, Ohkubo H: Augmented expression of atrial natriuretic polypeptide gene in ventricles of spontaneously hypertensive rats (SHR) and stroke-prone SHR. Circ Res 62: 926–930, 1988

    PubMed  Google Scholar 

  2. Hasegawa K, Fujiwara H, Doyama K, Miyamae M, Fujiwara T, Suga S, Mukoyama M, Nakao K, Imura H, Sasayama S: Ventricular expression of brain natriuretic peptide in hypertrophic cardiomyopathy. Circulation 88: 372–380, 1993

    PubMed  Google Scholar 

  3. Hasegawa K, Fujiwara H, Doyama K, Mukoyama M, Nakao K, Fujiwara T, Imura H, Kawai C: Ventricular expression of atrial and brain natriuretic peptides in dilated cardiomyopathy. Am J Pathol 142: 107–111, 1993

    PubMed  Google Scholar 

  4. Tanaka M, Hiroe M, Nishikawa T, Sato T, Marumo F: Cellular localization and structural characterization of natriuretic peptide-expressing ventricular myocytes from patients with dilated cardiomyopathy. J Histochem Cytochem 42: 1207–1214, 1994

    PubMed  Google Scholar 

  5. McGuire PG, Alexander SM: Inhibition of urokinase synthesis and cell surface binding alters the motile behavior of embryonic endocardial-derived mesenchymal cells in vitro. Development 118: 931–939, 1993

    PubMed  Google Scholar 

  6. Suzuki E, Hirata Y, Kohmoto O, Sugimoto T, Hayakawa H, Matsuoka H, Sugimoto T, Kojima M, Kangawa K, Minamino N: Cellular mechanisms for synthesis and secretion of atrial natriuretic peptide and brain natriuretic peptide in cultured rat atrial cells. Circ Res 71: 1039–1048, 1992

    PubMed  Google Scholar 

  7. Kinnunen P, Vuolteenaho O, Uusimaa P, Ruskoaho H: Passive mechanical stretch releases atrial natriuretic peptide from rat ventricular myocardium. Circ Res 70: 1244–11253, 1992

    PubMed  Google Scholar 

  8. Lakatta EG: Cardiovascular regulatory mechanisms in advanced age. Physiol Rev 73: 413–467, 1993

    PubMed  Google Scholar 

  9. Anversa P, Palackal T, Sonnenblick EH, Olivetti G, Meggs LG, Capasso JM: Myocyte cell loss and myocyte cellular hyperplasia in the hypertrophied aging rat heart. Circ Res 67: 871–885, 1990

    PubMed  Google Scholar 

  10. Gu J, McGrath LB: Localized endocrine conversion of ventricular cardiocytes in ventricular aneurysm. J Histochem Cytochem 38: 1659–1668, 1990

    PubMed  Google Scholar 

  11. Cantin M, Thibault G, Haile-Meskel H, Ding J, Milne RW, Ballak M, Charbonneau C, Nemer M, Drouin J, Garcia R: Atrial natriuretic factor in the impulse-conduction system of rat cardiac ventricles. Cell Tissue Res 256: 309–325, 1989

    PubMed  Google Scholar 

  12. DiNardo P, Minieri M, Carbone A, Maggiano N, Micheletti R, Peruzi G, Tallarida G: Myocardial expression of atrial natriuretic factor gene in early stages of hamster cardiomyopathy. Mol Cell Biochem 125: 179–192, 1993

    PubMed  Google Scholar 

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Raizada, V., Thakore, K., Luo, W. et al. Cardiac chamber-specific alterations of ANP and BNP expression with advancing age and with systemic hypertension. Mol Cell Biochem 216, 137–140 (2001). https://doi.org/10.1023/A:1011027231702

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  • DOI: https://doi.org/10.1023/A:1011027231702

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