Abstract
Angiotensin II (AGII) and thromboxane A2 (TXA2), potent vasoconstrictors, augmented the production of the precursor of tissue procollagenase/promatrixmetalloproteinase-1 (proMMP-1) and DNA synthesis in cultured human aortic smooth muscle cells (SMC) significantly compared with that in untreated SMC. Moreover, AGII and TXA2 stimulated hydrolysis of phosphoinositides and subsequent formation of inositol triphosphate (IP3), leading to an increase in the intracellular free Ca2+ concentration. These results suggest that the production of proMMP-1 increased by AGII and TXA2 in intimal SMC in relation to cell proliferation plays a role in arterial reconstruction in vascular diseases.
MeSH terms
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Angiotensin II / pharmacology*
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Aorta, Thoracic / cytology
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Aorta, Thoracic / drug effects
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Aorta, Thoracic / enzymology*
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Calcium / metabolism
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Cell Division / drug effects
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Cells, Cultured
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Collagenases / biosynthesis*
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DNA / biosynthesis
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Enzyme Precursors / biosynthesis*
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Humans
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Inositol 1,4,5-Trisphosphate / metabolism
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Kinetics
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Matrix Metalloproteinase 1
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Muscle, Smooth, Vascular / cytology
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Muscle, Smooth, Vascular / drug effects
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Muscle, Smooth, Vascular / enzymology*
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Thromboxane A2 / pharmacology*
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Thymidine / metabolism
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Vascular Diseases / pathology
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Vascular Diseases / physiopathology
Substances
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Enzyme Precursors
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Angiotensin II
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Thromboxane A2
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Inositol 1,4,5-Trisphosphate
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DNA
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Collagenases
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Matrix Metalloproteinase 1
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Calcium
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Thymidine