Using sonoreflective endovascular targets of known length (stainless steel tubular slotted stents), we have validated in vivo the accuracy and reproducibility of intravascular ultrasound length measurements using a system incorporating motorized transducer pullback through a stationary imaging sheath. The correlation was r = 0.936, with a measurement error of only +/- 5.2%, minimal intraobserver variability, and variability of sequential measurements of only +/- 4.8%.