You are here

Article Text

Article menu
Download PDFPDF
Basic and Translational Medicine
Basic research of cardiovascular disease
GW24-e1353 Effects of N-carboxymethyl-Lysine on migration from RAW264.7-derived foam cells
  1. Wang Zhongqun1,
  2. Li Lihua2,
  3. Yan Jinchuan1
  1. 1Department of Cardiovascular Diseases, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China
  2. 2Department of Pathology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China

Abstract

Objectives To investigate the effects of Nε-carboxymethyl-Lysine (CML), a key bioactive components of AGEs, on migration from RAW264.7-derived foam cells.

Methods The transmembrane migration ability was assessed by Boyden chamber cell migration experiment in vitro. The intracellular accumulation of free cholesterol, cholesterol ester and total cholesterol was determined by cholesterol oxidase methods. The expression of CD36 mRNA and protein was analysed by RT-PCR and Westernblot.

Results 10 μmol/L CML significantly promoted the intracellular accumulation of free cholesterol, cholesterol ester and total cholesterol in RAW264.7-derived foam cells, upregulated the expression of CD36 mRNA and protein, inhibited the transmembrane migration ability of foam cells (The mean fluorescence intensity of migrated foam cells in CML + oxLDL group was reduced by 59.79%, compared with oxLDL group; It was reduced by 73.46%, compared with control group.).

Conclusions CML may initiate CD36 cascade by cooperating with oxLDL and result in the inhibition of the transmembrane migration ability.

https://doi.org/10.1136/heartjnl-2013-304613.135

Statistics from Altmetric.com

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.